Ultra-Violet/Visible (UV/VIS) Spectrophotometry
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Scope:
UV/VIS Spectrophotometry is used to determine the absorption or transmission of UV/VIS light (180 to 820 nm) by a sample. It can also be used to measure concentrations of absorbing materials based on developed calibration curves of the material.
Test Procedure:
A sample is placed in the UV/VIS beam and a graph of the transmittance or absorbance versus the wavelength is obtained.
Alternatively, samples are prepared in known concentrations and their absorbance is read by the UV/VIS Spectrophotometer. Results are then graphed to make a calibration curve from which the unknown concentration can be determined by its absorbance.
Specimen size:
Transparent plastics or films.
Solutions of transparent solids.
Data:
A spectrum of percent transmittance or absorbance versus wavelength.
The concentration can be found by mathematical methods based on Beer’s Law
Equipment Used:
Hewlett Packard Diode Array UV/VIS Spectrophotometer
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